Pricing & Turnaround

The most inexpensive way to do DNA sequencing! Whether you just don't want the bother of getting a new purchase order every time, you want to "bank" some grant money or you want to take advantage of the great savings available, our spend-down accounts are just what you need. Due to the nature of our work, we can only apply the substantial cost-savings to our basic* DNA Sequencing reactions, however spend-down accounts can be applied to ALL our services.

*Unfortunately, due to the complexity of GC-rich templates, BAC/PAC/YAC clones and our 96-well plate service, we cannot offer additional savings on these.

How to Purchase Spend-Down Account

• Please simply purchase a set number of reactions online when setting up your account
• The corresponding amount of credit will show up for your account

Using your Spend-Down Account

• Upon initiating the order process, when paying, just select ‘Use Spend-Down Account’
• The number of reactions purchased will be deducted from your account, accordingly

Pricing

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to DNA Sequencing and Services' Terms and Conditions.

Advantages

• Completely flexible. You send any type (tubes/plates) and number of samples as and when you want.
• No fiddly vouchers to keep track of!
• We will notify you as your account nears its end – you can easily "top up" your account online when needed.

Turnaround

Turnaround times will depend on the precise service chosen. Please review the details of this in the specific Sequencing Reaction section below.

Standard Pricing

Reactions

• *Quant-It: We are happy to quantitate the concentration of your template for only £1
• ^Augmented Protocol: If your template has known secondary structure or a high GC content, £0.50 will be applied per reaction to facilitate the sequencing reaction using an 'Augmented Protocol'

Primer

• Standard primers offered by MRC PPU DNA Sequencing and Services are free!
• Custom primer synthesis prices will reflect the size of the primer – the cost to the customer is £0.50 per base

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

We perform the cycle sequencing reactions on the day the samples are supplied to us, followed by evaluation of productions on our sequencers. Results are routinely supplied to customers within 24 hours upon receipt of primer and template.

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Reactions

• *Quant-It: Please note that we are unable to offer the Quant-It service when PCR products are used as templates. This is because we use a Nanodrop spectrophotometer to determine DNA concentrations of templates that are provided to us and the use of spectrophotometric analysis for determining PCR product concentrations is often unreliable due to the presence of other PCR components (e.g. primers, dNTPs, background products). This can even be the case with "purified" products, depending on how clean they are. Customers should also note that purification of PCR products can be unnecessary or even detrimental to the sequencing reaction. Please take a look at our dedicated help page on sequencing PCR products for more information.
• ^Augmented Protocol: If your template has known secondary structure or a high GC content, £0.50 will be applied per reaction to facilitate the sequencing reaction using an 'Augmented Protocol'

Primer

• Standard primers offered by MRC PPU DNA Sequencing and Services are free!
• Custom primer synthesis prices will reflect the size of the primer – the cost to the customer is £0.50 per base

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

We perform the cycle sequencing reactions on the day the samples are supplied to us, followed by evaluation of productions on our sequencers. Results are routinely supplied to customers within 24 hours upon receipt of primer and template.

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FOR STANDARD PLASMIDS:  If all your templates are of a similar composition (<20kb and do not contain secondary structure/high GC-content) each sample on a 96-well plate can be processed at a cost of £4.50 per well

FOR PLASMIDS WITH HIGH GC-CONTENT:  If all your templates are of a similar composition (<20kb, containing secondary structure/high GC-content) each sample on a 96-well plate can be processed at a cost of £5.50 per well. This is due to the fact that an 'Augmented Protocol' will be used for plamids known to contain secondary structure/high GC-content.

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

We perform the cycle sequencing reactions on the day the plates are supplied to us, followed by evaluation of productions on our sequencers. Results are routinely supplied to customers within 24 hours upon receipt of plates.

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Reactions

• *Quant-It: We are happy to quantitate the concentration of your template for only £1
• ^Augmented Protocol: If your template has known secondary structure or a high GC content, £0.50 will be applied per reaction to facilitate the sequencing reaction using an 'Augmented Protocol'

Primer

• Standard primers offered by MRC PPU DNA Sequencing & Services are free!
• Custom primer synthesis will reflect the size of the primer – the cost to the customer is £0.50 per base

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

To achieve optimal results with such large templates, we have to modify our standard sequencing conditions and so these samples have to be processed separately. We routinely perform the cycle sequencing reactions on the day the samples are supplied to us, followed by evaluation of productions on our sequencers.   

Given the specialised nature of these samples, please understand that turnaround times may be slightly longer than for standard-sized templates.

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STANDARD EST SEQUENCES:  If all your templates are of a similar composition (<20kb and do NOT contain secondary structure/high GC-content) each EST can be processed for the following costs:

• Processing of ESTs includes:
  • Streaking of colonies
  • Isolation of 4 colonies
  • Extraction of 4 colonies/mini-prep growth
  • Sequencing of up to 2 separate colonies

FOR PLASMIDS WITH HIGH GC-CONTENT:  If all your templates are of a similar composition (<20kb, containing secondary structure/high GC-content) each EST can be processed at the costs indicated below. This is due to the fact that an 'Augmented Protocol' will be used for plasmids known to contain secondary structure/high GC-content.

• Processing of ESTs includes:
  • Streaking of colonies
  • Isolation of 4 colonies
  • Extraction of 4 colonies/mini-prep growth
  • Sequencing of up to 2 separate colonies

Primer

• Standard primers offered by MRC PPU DNA Sequencing & Services are free!
• Custom primer synthesis prices will reflect the size of the primer – the cost to the customer is £0.50 per base

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

Turnaround time for this option will be highly dependent on receipt of a customer’s EST clone and as well as the requisite number of days needed for each of the steps outlined above. We can routinely provide sequence information of ESTs (utilizing standard primers) within 4 working days of receipt of the clone. 

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Primer

• Custom primer synthesis prices will reflect the size of the primer – the cost to the customer is £0.50 per base and includes the following services:
  • Ordering of custom primer (upon receipt of sequence from customer)
  • Assessment of concentration and purity of primer
  • Diluting primer to appropriate concentration for reaction
  • Storage of primer on file and in facility freezer for 1 year

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

Though we do not make the primers ourselves, we do utilise a supplier who has historically demonstrated rapid turnaround of synthesis and shipment. However, please note that delays can occur from time to time. Should there be a delay that we are made aware of, we will inform you as soon as we can.

Standard Pricing

Please note that sequencing of minipreps requires quantification of DNA concentration and thus £1 is included in the pricing

• ^Augmented Protocol: If your template has known secondary structure or a high GC content, £0.50 will be applied per reaction to facilitate the sequencing reaction using an 'Augmented Protocol'

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

Many of our customers prefer to take care of the steps involving choice of bacterial colony and growth of culture. Thus from the point of a centrifuged pellet, our robots routinely purify 96 bacterial plasmid DNAs in two hours with minimal intervention from staff.

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Standard Pricing

Please note that our standard midiprep service includes quantification of DNA concentration

• ^Augmented Protocol: If your template has known secondary structure or a high GC content, £0.50 will be applied per reaction to facilitate the sequencing reaction using an 'Augmented Protocol'

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

Starting from an already grown culture, our facility can routinely turn samples around within one day of receipt. Additional time will be needed should plasmid DNA be submitted for the individual required steps (transformation, culture growth).

Customers are asked to add one day to those requests for DNA Sequencing & Services with a starter culture – to allow for amplification of bacteria/plasmid. 

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Standard Pricing

Please note that our standard maxiprep service includes quantification of DNA concentration

• ^Augmented Protocol: If your template has known secondary structure or a high GC content, £0.50 will be applied per reaction to facilitate the sequencing reaction using an 'Augmented Protocol'

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

Starting from an already grown culture, our facility can routinely turn samples around within one day of receipt. Additional time will be needed should plasmid DNA be submitted for the individual required steps (transformation, culture growth).

Customers are asked to add one day to those requests for DNA Sequencing & Services with a starter culture – to allow for amplification of bacteria/plasmid. 

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Standard Pricing

Fragment Analysis Alone

• 48-sample Plate: £48
• 96-sample Plate: £96

Please note that prices are exclusive of VAT, which customers outside The University of Dundee will be charged at the appropriate rate (currently 20%) on all products and that all sales are subject to MRC PPU DNA Sequencing and Services' Terms and Conditions.

Turnaround

We can evaluate your samples on our capillaries upon receipt of 96-well plates and final fragment analysis results are routinely supplied to customers within 24 hours.

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